I did get agglutination when I react HCl with silver nitrate (recipe from online). However the agglutination is white colour in a pool of red water.
If you all seen a real blood grouping before using blood and the serum with different antibodies, the agglutination is actually red in a pool of pale yellow solution (which is serum). Below are my recipe:
Blood Gp A - Star brand Red Dye + Tap water
Blood Gp B - 10% FeCl3 (Iron III Chloride) + Char Siew powder (to give red colour)
Blood Gp O - Char Siew powder + Tap Water
* Star brand Green dye is used to adjust the red colour to look more like blood.
Anti A - 0.1M HCl (Hydrochloric Acid)
Anti B - 0.1M NaOH (Aq Sodium Hydroxide)
So after a few trial with different chemical, I finally come out with my own recipe. All thanks to Star brand red dye which agglutinate in the presence of acid.
However I do have a problem is that I cannot make Blood AB as the Red dye (from Blood A) will agglutinate in the presence of FeCl3 which is acidic even no acid is added (I only mixed 5g of FeCl3 powder with 50ml of tap water and it gives a pH of about 1.5)
So I intend to leave out Blood AB, as after a lot of trial, I still cannot get the result I want. I even let the agglutination in my Blood AB to settle down and get the supernatant. However it only react with Anti B (NaOH) but not in Anti A (HCl) which I think all have been reacted to form agglutination so the supernatant does not gives a reaction when react with Anti A (HCl).
If anyone who read my article can create Blood AB, or you have any recipe where the result is as good as mine shown in the picture (really look like real life blood agglutination). Please leave comment in my blog. Thank you.
Attached below is the picture of the item I used for my blood grouping kit.
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